Shadab Habibi Email & Phone Number

I am a biochemist who has a strong passionate about learning new skills . SKILLS Protein skills: Protein purification, preparative column chromatography, membrane filtration, dialysis, SDS-PAGE, Western blot, bacterial protein expression, protein refolding, mammalian cell protein expression and ELISA.Molecular Biology skills: Plasmid preparation, ligation, bacterial and yeast transformation, Genomic DNA extraction from bacteria, virus, cultured cells and whole blood cells, total RNA extractionfrom different samples, probe and primer designing,real-time PCR, PCR(RT-PCR, NestedPCR, touch down PCR and site-directed mutagenesis).Cell Biology skills: generation, and maintenance of cell cultures (mouse and human),Transfection(human and mouse stem cell and human fibroblast cell and normal cell line like hek293T and CHO cells), luciferase reporter assay, cell counting (haemacytometer), Flow cytometry, ATP and ADP assay, cell viability assay (MTT assay), caspase assay, stable cell line generation (by viral vector system), fluorescent microscopy, cell cloning, cell proliferation assay and cell culture