Cell Culture Ops at Global Raw Materials and Reagents, Centre of Excellence.

Developed new assays for raw material testing.Material qualification for new products.Quality records.

* Marijuana Smoke Promotes Dysfunctional Macrophage Response to HIV and Pneumonia :Working with blood and bronchial lavage of human subjects who are heavy Marijuana users and comparing the immune and metabolic status to non-smokers or tobacco smokers.

* New insights of microenvironment signaling by association of Cancer Stem Cells with Extracellular matrix proteins. * Evaluated peptidomimetics & fusion Abs as therapeutics targets for novel nanodrug development. * Assessed novel nanodrugs for toxicity, treatment breast & brain tumors to impede tumor-associated signaling. * Evaluated theranostic nanodrugs for noninvasive diagnostic imaging, Alzheimer's imaging by microMRI. * Studied effects of air pollution on brain & gene expression changes by microfluidic qPCR. * Evaluated role of CK2 in glioblastoma.* 4+ years of experience with IACUC writing. IACUC & IRB certified.* Extensive experience with specialized analysis softwares like Fluidigm, Prism, MS Office, Image Studio, and manuscript writing.

Experience in basic and clinical research in development of adaptive cellular immunity in humans to Hep B vaccination.• Evaluating the impact of habitual Marijuana use on humoral and cellular immune responses to a Hepatitis B virus vaccine.• Gene analysis of dendritic cells upon delta-9-tetrahydro cannabinoid exposure by gene array.• Studies on delta-9-Tetrahydrocannabinoid (THC) induced-Cannabinoid Receptor 2-mediated apoptosis. • Extensive experience of working with clinical protocols, human blood sample ficoll density separation, multicolor flow cytometry, intracellular cytokine detection, T-cell proliferation.• Experience working with FDA controlled substances. • Been part of data generation, analysis and manuscript writing.

Experience in basic science research in autoimmunity.• Spearheaded the study to characterize autoimmune T cells in lupus prone mice particularly cytokine production, activation status, and phosphorylation kinetics. • Characterized the phenotype of CD4 T cells in murine models of lupus with regard to effector/memory population, differential cytokine production and important surface markers.• Study of vitamin D receptor pathway as a putative biomarker for lupus nephritis• Involved in project to study defective regulatory T cell function in lupus prone mice. • Study protective role of Langerhans cell migration in lupus prone mice. • Part of study involving role of type 2 cytokines in scleroderma in human subjects.• Experience with analysis of microarray data, creating heat maps of genes of interest.• Been part of data generation, and analysis and experience in grant and manuscript writing.• Actively participated and presented scientific manuscripts in ‘Translational Immunology Journal Club’ and various other weekly journal / data clubs, National and International meetings.

Established a ELISA method to detect mild and strong strains of citrus viruses.

Worked on detecting and establishing mild strains of citrus virus for cross protection technology. Performed eye and stem transplants in citrus plants.