* Test protein concentration assay to determine concentration of protein to use for a PKCepsilon ELISA assay that can test for the presence of Alzheimer’s disease human skin fibroblast cells. * Perform cell culture of human skin fibroblast cells. * Use confocal microscopy to make Z-Stack images for determining the amount of * phosphorylation of ERK1 and ERK2 and measure bradykinin stimulation in human cell culture samples to test for the presence of Alzheimer’s disease.

* Test protein concentration assay to determine concentration of protein to use for a PKCepsilon ELISA assay that can test for the presence of Alzheimer’s disease human skin fibroblast cells. * Perform cell culture of human skin fibroblast cells. * Use confocal microscopy to make Z-Stack images for determining the amount of * phosphorylation of ERK1 and ERK2 and measure bradykinin stimulation in human cell culture samples to test for the presence of Alzheimer’s disease.

* Performed testing of the reagents against the specifications for uniformity and component testing when testing kits that where made by the pilot plant. * Developed, executed and analyzed data from verification protocols on DNA Sample Preparation reagents using the Qiasymphony DNA extraction automated platform. * Scaled up reagents and developed Master manufacturing records (MMR) documentation for reagents used for a bead-based hybrid capture assay (ELISA assay). * Developed reagent in process quality controls using pH and spectrophotometry. * Performed internal manufacturing using cGMP and GLP methods for a bead based an IVD hybrid capture assay. * Developed stability documentation according to FDA and CE marking regulations and executed manual testing for a magnetic bead based IVD hybrid capture assay. * Performed multiplex experiments using Luminex technology and utilized qPCR for result verification from the Luminex results. * Maintained laboratory notebooks and gave presentations to senior leadership personnel on the status of stability studies.

* Created paperwork for processing samples, corresponding with information provided by sponsors. * Performed DNA and RNA extraction using a physical extraction method with Qiagen reagents. * Performed real-time PCR, RT-PCR and whole genome amplification using the BIO-RAD IQ systems. * Performed procedures using SOPs, GLP, and aseptic techniques. Analyzed PCR data, made reports, and performed quality control analysis prior to data being delivered to client. Performed inventory and purchased reagents and equipment needed for lab operation.

* Performed quality control on molecular biology products using PCR, RT-PCR, fluorometer, refractometry, pH, conductivity, and competent cells. * Developed quality control documents and methods for testing products, determined whether products would pass or fail. * Made data packets and filed quality control documents for competent cells and PCR product analysis.

* Performed Electrochemluisence (ECL) assays and validation maintenance on several machines while keeping logs of reagents used and ECL signals that were produced. * Performed physical and chemical DNA extraction using Qiagen kits and specially designed buffers to lysis cells. * Ran real-time PCR assays using lyophilized and wet reagents for both the RAPID and ABI real time PCR detection equipment. * Assisted in plating and identifying possible biological agents using various isolation techniques.